Figure 3
From: Fluorescent enzyme-coupled activity assay for phenylalanine ammonia-lyases
(a) Fluorescence signal turn-on at different styrene 3 and fluoroprobe 4 concentrations at the corresponding excitation and emission maxima determined by 3D excitation-emission scans, considering also the background fluorescence (see also Supplementary Fig. S5, S6). (b) and (c) The detection limit (defined as mean of negative controls plus three times the standard deviation of negative controls) of styrene is 25 μM, while in presence of 0.5 mM of fluorogenic probe 4 linear response occurs for 25 μM-0.1 mM styrene concentration (see also Supplementary Fig. S7).
