Table 1 The relative activity order of different PcPAL-variants obtained by the fluorescence-assay in comparison with their activity order obtained through HPLC- and UV-based activity measurements.

From: Fluorescent enzyme-coupled activity assay for phenylalanine ammonia-lyases

PcPAL variant

Relative activities (%)

Fluorescence assaya

HPLC assayb

UV290nm-assayc

I460A

100

100

16.2

I460V

84.7

94.8

100

F137V

64.3

89.7

14.0

wild-type

14.3

7.7

2.5

L138V

6.0

7.7

<1

  1. aDetermined from the relative fluorescence signal intensities; bBased on the relative conversions values of ammonia elimination reactions after 16, using whole cell PAL-biocatalysts (see Materials and methods); cDetermined by UV290nm-assay monitoring the p-MeO-cinnamic acid production, using the corresponding purified PAL enzymes (see Materials and methods).
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