Figure 3 | Scientific Reports

Figure 3

From: Targeting of the E3 ubiquitin-protein ligase HUWE1 impairs DNA repair capacity and tumor growth in preclinical multiple myeloma models

Figure 3

HUWE1 knockdown reduces tumour growth of xenotransplanted MM1.S-shHUWE1 cells in NSG mice in vivo. (a) In vivo imaging of tumour load 7 and 14 days after MM1.S cell transplantation. The cells were injected into the tail vein of the animals, and they were separated into control (n = 5) and HUWE1kd groups (n = 5). HUWE1 knockdown was induced with doxycycline (200 mg/kg) containing diet. (b) Tumor growth was measured by counting emitted photons/sec in the animals without (HUWE1kd, white symbol) and with HUWE1 expression (control, black symbol) in the luciferase expressing MM1.S cells after i.p. luciferin injection. Images were recorded using Living Image software, version 4.4 (Perkin Elmer, Waltham, USA).

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