Figure 4

Histomorphometric analysis of bone regeneration at 6 weeks after implantation was performed by using the ImageJ software version 1.50i. (A) The results reported as percentage of total area show that the defect coverage was complete for BMP4 and FGF2/ VEGF/ BMP4 groups. (B) The ratio of cartilage tissue to coverage defect was significantly increased in the VEGF group compared to control C, BMP4 and FGF2/ VEGF/ BMP4 groups. (C) The amount of new bone area reported to coverage defect was higher in the FGF2/ VEGF/ BMP4 group compared to the other conditions. Unpaired 2-tailed Student’s t test was used. *p < 0.05, ***p < 0.001 vs C; ^$p < 0.05, ^$$p < 0.01 vs BMP4; ^#p < 0.05, ^##p < 0.01; ^###p < 0.001 vs FGF2/ VEGF/ BMP4; √√p < 0.01vs FGF2/ VEGF.