Figure 1 | Scientific Reports

Figure 1

From: Enhanced FGFR3 activity in postmitotic principal neurons during brain development results in cortical dysplasia and axonal tract abnormality

Figure 1

Expressing FGFR3K650E in NEX-lineage neurons results in aberrant cortical lamination. (A) The diagram shows how GOF mice were generated. The black triangle indicates the loxP site. (B) Western blots show the abundance of phosphorylated FGFR substrate 2 α (FRS2α-Tyr196; pFRS2α), FRS2α, phosphorylated ERK1/2 (Thr202/Tyr204; pERK1/2), ERK1/2 in the S1 cortex of P7 control (Ctrl) and GOF mice. (C) Summaries for the fold changes in pFRS2α to FRS2α and pERK1/2 to ERK1/2 (Ctrl, n = 5; GOF, n = 6) in GOF mice. (D,E) VGluT2, NeuN, and DAPI staining in the P7 S1 cortex. D1-3 and E1-3 show the enlarged images in (D,E) (yellow boxes). D2-3 and E2-3 show the inverted images of individual channels. I–VI, cortical layers; SP, subplate. Student’s-t test. *, p < 0.05; ***, p < 0.001. See Supplementary Fig. 10 for full-length blots.

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