Figure 1 | Scientific Reports

Figure 1

From: Impaired mitochondrial medium-chain fatty acid oxidation drives periportal macrovesicular steatosis in sirtuin-5 knockout mice

Figure 1

Sirt5KO mice develop hepatic macrovesicular steatosis on coconut oil diet. (A) Representative hematoxylin and eosin liver tissue staining from wild-type and Sirt5KO mice maintained on four different diets for five weeks: standard diet (SD), high-fat C8/C10 diet, high-fat coconut oil diet (Coco), or high-fat long-chain triglyceride (LCT) diet. The relative composition of these diets is illustrated in Supplemental Fig. 1. Fat droplets were visible in only coconut-fed Sirt5KO mice. Scale bar, 100 µM. (B) Inset from coconut-fed Sirt5KO liver in panel (A), showing lipid droplets at a higher resolution. (C) Liver triglyceride content of mice maintained on the four diets for five weeks. N = 6 for C8/C10 and LCT diets, and N = 10 for coconut and SD diets. There was no gender difference in triglyceride accumulation; all groups contained half males and half females, age 6–8 weeks at time of diet onset. *P < 0.01 Sirt5KO versus wild-type controls.

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