Figure 6
Omega oxidation is upregulated in Sirt5KO livers. (A) Immunoblotting for peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (EHHADH) in standard diet (SD) and coconut-fed (Coco) wild-type and Sirt5KO livers, N = 4. β-actin served as loading control. The uncropped versions of these blots appear in Supplemental Fig. 4. (B) Densitometric analysis of panel (A). *P < 0.01 for indicated pairwise comparisons. (C) Urine adipic acid, the peroxisomally-generated product from degradation of the dicarboxylic acids produced by omega oxidation; N = 3 wild-type (WT) and Sirt5KO coconut-fed mice. *P < 0.01. (D) Flux of 14C-dicarboxylic (DC) C12 to labeled acid-soluble metabolites in cultured mouse embryonic fibroblasts (MEFs), N = 4 replicates. *P < 0.05.
