Figure 1

Reduced SASH1 mRNA expression is a prognostic indicator for poor lung cancer patient survival. (A–D) Box plots generated from The Cancer Genome Atlas (TCGA) RNAseq lung cancer datasets of overall SASH1 expression in lung tumor and normal tissue (A), stages of disease (B), adenocarcinoma subsets (C) and squamous cell carcinoma subsets (D). Shown are the median expression levels with 95% confidence intervals (notches), interquartile ranges (boxes) and all data points (grey dots). P values: Mann–Whitney U tests compared to expression in normal tissues. Sample sizes (n) are shown above the x-axes. (E–G) Univariate Kaplan–Meier analysis of overall survival using Medium expression. Overall survival was assessed in all NSCLC cases (E), adenocarcinoma (F) and squamous cell carcinoma (G). SASH1 protein expression was assessed using the protein atlas data, expression was shown to be low in lung carcinoma (H–I) using the same anti-SASH1 Antibody used in this study (HPA029947). SASH1 mRNA and protein levels were compared in 77 lung cancer cell lines (J). (A–D) were generated in the R computing environment (version 4.0, R Project for Statistical Computing, Vienna, Austria, https://www.r-project.org).