Figure 3

SASH1 protein levels can mediate cisplatin sensitivity. (A–F) SASH1 depletion with esiRNA in lung cancer cells confers resistance to cisplatin. Cell were seeded at equal density 48 h post depletion of SASH1 and treated with cisplatin at indicated doses (1–10 μM) 6 h post seeding. Cell survival was measured 48 h following cisplatin treatment. (G–L) SASH1 overexpression results in decreased cell proliferation with an additive effect from cisplatin treatment. Cells were transfected with SASH1-Flag or Flag alone (Control) and seeded 24 h post transfection cells where treated with cisplatin at IC30 concentrations 6 h post seeding. (M) Immunoblot of SASH1 overexpression in lung cancer cells from (G–L) indicating SASH1 expression.