Figure 2 | Scientific Reports

Figure 2

From: Increased expression of LCN2 formed a positive feedback loop with activation of the ERK pathway in human kidney cells during kidney stone formation

Figure 2

Oxalate induced high expression of LCN2 in renal epithelial cells. HK-2 (A) and HEK-293 (B) were treated with indicated concentrations of oxalate. The cell viability was determined and analyzed. HK-2 (C) and HEK-293 (D) were treated with 1 mM oxalate for indicated time. The expression of LCN2 was analyzed by RT-PCR. HK-2 (E) and HEK-293 (F) were treated with 1 mM oxalate for indicated time. The expressions of LCN2 and GAPDH were analyzed by western blot. (G) The quantitative results of bands in the E and F. All experiments were repeated at least three times. (*P < 0.05, **P < 0.01, ***P < 0.001).

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