Figure 6 | Scientific Reports

Figure 6

From: Increased expression of LCN2 formed a positive feedback loop with activation of the ERK pathway in human kidney cells during kidney stone formation

Figure 6

The level of pERK1/2 was affected by LCN2. HK-2 (A) and HEK-293 (B) were transfected with negative control (NC) siRNA or LCN2 siRNA 1/2/3, and the expressions of LCN2 and GAPDH were analyzed by western blot. (C) HK-2 and HEK-293 were transfected with NC siRNA or LCN2 siRNA1/2/3 pools, and the expressions of p-ERK1/2, ERK1/2, LCN2 and GAPDH were analyzed by western blot. (D) HK-2 and HEK-293 were transfected with vector or LCN2, and the expressions of p-ERK1/2, ERK1/2, LCN2 and GAPDH were analyzed by western blot. HK-2 (E) and HEK-293 (F) were treated with rhLCN2, and the expressions of p-ERK1/2 and ERK1/2 were analyzed by western blot. All experiments were repeated at least three times.

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