Figure 3
From: IL-18 binding protein (IL-18BP) as a novel radiation countermeasure after radiation exposure in mice
Treatment with IL-18BP decreased free IL-18 levels in mouse serum. Mice received single dose of rhIL-18BP (2 mg/kg) or vehicle-control SC injection 48 h post-9.4 Gy TBI and serum were collected on day 3 and 4 after TBI. Levels of mouse IL-18, endogenous mouse IL-18BP and exogenous human IL-18BP in mouse serum were measured with corresponding ELISA kits. (a) The total levels of IL-18BP (endogenous mouse IL-18BP and exogenous human IL-18BP) in vehicle-treated or rhIL-18BP-treated mouse serum and (b) free IL-18 in mouse serum. Data of total IL-18 in serum and free IL-18 levels by calculation in vehicle treated and IL-18BP treated group 3 and 4 day after TBI are included. Results were obtained from two independent experiments (6 mice/group/experiment, N = 12). Means ± SD. *p < 0.05; **p < 0.01; IL-18BP-treated vs. vehicle-treated.
