Figure 3 | Scientific Reports

Figure 3

From: A cell-based bioluminescence assay reveals dose-dependent and contextual repression of AP-1-driven gene expression by BACH2

Figure 3

Dose-dependent repression of AP-1-driven gene expression by BACH2. (a) Luciferase activity of inducible-BACH2 reporter line after 6 h PMA/ionomycin stimulation with or without pre-treatment with indicated titrated doses of tetracycline. (b) Western blot analysis of the abundance of BACH2 protein within total protein lysates from cells in (a). Quantified and normalised to β-actin levels of BACH2 protein expression are displayed in the bar graph (top). (c) Positive correlation between BACH2 expression normalised to β-actin, and luciferase signal repression at the indicated in (a) tetracycline concentrations. Two-way ANOVA with Bonferroni correction (a) and linear regression analysis (c). Data are representative of 2 independently repeated experiments with 3 culture replicates per condition. Bars and error represent mean (SD); ns not significant; ***P < 0.001; ****P < 0.0001.

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