Figure 3
From: Comparative physiological and transcriptomic analysis of pear leaves under distinct training systems
Global view of the RNA-seq expression data at three growth stages in pear. (A) Overview of gene expression in different samples. Samples from the SP (traditional freestanding system) and DP (flat-type trellis system) were collected at 45 DAF (day after flowering), 75 DAF and 105 DAF. FPKM: Fragments Per Kilobases per Millionreads. The white, grey and dark segments in each stacked bar indicate the distribution of different expression levels. (B) Hierarchical clustering analysis of all expressed genes across the different samples. Each column and row corresponds to one sample. Color gradient from red-to-blue indicates similarity degree change from high to low. (C) The total number of up- and down-regulated genes at three growth stages. (D) Venn diagram showing the number of genes with differentially expressed levels between SP and DP systems at the three growth stages. (E) Verification of RNA-seq results by qRT-PCR. Error bars indicate the standard deviation from three biological and technical replicates of qRT-PCR analysis. The Y-axises show the relative gene expression level analyzed by qRT-PCR (blue bars, left) and corresponding expression data of RNA-seq (red line, right).
