Figure 6 | Scientific Reports

Figure 6

From: Reconstitution of rat fetal testis during the masculinisation programming window induces focal dysgenesis consistent with testicular dysgenesis syndrome

Figure 6

Leydig cells are present in re-aggregated rat fetal testis xenotransplants although seminal vesicle weight is reduced in host mice exposed to DBP. (A) Seminal vesicle weight and (B) serum testosterone in xenotransplanted host mice (n = 5–6) exposed to vehicle (VEH) control or DBP. Each data point represents total transplant weight obtained from a single host mouse. Statistical analysis performed using Mann–Whitney test. Bar represents median value. * p < 0.05. (C–F) Triple immunofluorescence for SMA (yellow; basement membrane) and SOX9 (green; Sertoli cells) and 3β-HSD (red: Leydig cells) in vehicle- (VEH) and DBP-exposed xenotransplants, demonstrating Leydig cell aggregates (*) and individual Leydig cells (arrows). Scale bar = 50 µm.

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