Figure 7 | Scientific Reports

Figure 7

From: Reconstitution of rat fetal testis during the masculinisation programming window induces focal dysgenesis consistent with testicular dysgenesis syndrome

Figure 7

Intratubular Leydig cells are present in re-aggregated rat fetal testis xenotransplants. (A–D) Triple immunofluorescence for SMA (yellow; basement membrane) and SOX9 (green; Sertoli cells) and 3β-HSD (red: Leydig cells) in vehicle- (VEH) and DBP-exposed xenotransplants, demonstrating individual ITLCs (arrows; A,B) and ITLC clusters (arrowheads; C,D). Scale bar = 50 µm. (E) High magnification of area indicated by box in (D) showing ITLC. (F) ITLCs in xenotransplants from host mice exposed to vehicle (VEH) control or DBP. Each data point represents an individual xenotransplant. Statistical analysis performed using students t-test. Values are mean ± SEM per group for n = 5.

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