Figure 1
Mouse survival and cytokine expression in the lungs of influenza convalescent mice after airway exposure to Kim53. (A) Changes in body weight of mice inoculated i.n. with the PR8 mouse-adapted influenza virus (20 pfu) compare to naïve (n = 24 for mice 25 dpii and n = 9 for naïve mice) ***p < 0.001 vs. naïve. Lower panel, experimental setting scheme of Kim53 infection at different time points postinfluenza. (B) Kaplan–Meier survival curves of mice i.n. infected with a sublethal dose (0.2 LD50) of the fully virulent Y. pestis strain Kim53 at different time points postinfluenza (n = 38 for naïve mice, n = 24 for mice 25 dpii, n = 5 for mice infected with Kim53 at 7, 10, 14 and 40 dpii). (C) Cytokine and chemokine levels in the lungs at the indicated time points after i.n. infection of naïve mice with 0.2 LD50 of Kim53 (black) compared to mice infected with a similar dose of Kim53 administered at 25 dpii (blue) (n = 7–8 mice per time point,). #p < 0.05, ###p < 0.001 vs. Kim53 at t = 0. *p < 0.05, **p < 0.01, ***p < 0.001 vs Kim53 at the same time point.
