Table 1 Summary of clinical and histopathological data.

	Breed	Sex	Age (years)	Tumor location	Size (cm)	Grade		Lymph node status^e
	Breed	Sex	Age (years)	Tumor location	Size (cm)	Patnaik	Kiupel	Lymph node status^e
1	American Staffordshire Terrier^a,d	Male	6	Trunk	1	II	Low	HN1
2	Bernese	Female	4	Limb	2.5	II	Low	HN2
3	Boxer	Male	8	Limb	2.2	II	Low	HN2
4	Dachshund	Female	9	Trunk	0.8	II	Low	HN0
5	Dogo^a,b	Male	6	Limb	2	II	Low	HN2
6	English setter^a,b	Female	6	Trunk	3	II	Low	HN2
7	English setter^a,c,d	Female	11	Trunk	5	II	Low	HN3
8	Italian pointer^a,b	Male	5.5	Trunk	4	II	Low	HN1
9	Jack Russell^a,b	Male	13	Head	5	II	High	HN2
10	Labrador	Male	1	Head	1	I	Low	HN0
11	Labrador ^d	Male	10	Scrotum	2	II	Low	HN0
12	Labrador	Male	9	Trunk	0.6	II	Low	HN2
13	Labrador	Female	6	Trunk	3.5	II	Low	HN2
14	Mixed breed^a,b,c	Female	11	Trunk	3	II	Low	HN0
15	Mixed breed^a,b	Female	6	Trunk	4	II	Low	HN2
16	Mixed breed^a,b	Male	11	Limb	3	III	High	HN2
17	Mixed breed^a,c,d	Female	8	Limb	3	III	High	–
18	Mixed breed^c	Male	12	Neck	7	II	Low	HN3
19	Pug	Male	3.5	Head	1	II	Low	HN2
20	Tosa inu	Male	4	Trunk	3	II	Low	HN2
21	Weimaraner	Male	7	trunk	2	II	Low	HN2

^aMCT samples sequenced using NGS.
^bHealthy margins sequenced using NGS.
^c$amples in which miRNAs selected for the RT-qPCR validation step were not detected.
^dSamples for which healthy margins were not collected.
^eClassification system proposed by Weishaar et al.⁷. HN histological node, NGS next-generation sequencing.

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