Table 1 Oligonucleotides sequences, target genes, specific amplicon size, and PCR re-cycling conditions.

Target gene	Primers sequences	Amplicon size (bp)	Amplification (35 cycles)			References
Target gene	Primers sequences	Amplicon size (bp)	Denaturation	Annealing	Extension	References
lt	GGTTTCTGCGTTAGGTGGAA	605	94 °C 30 s	57 °C 45 s	72 °C 45 s	²³
lt	GGGACTTCGACCTGAAATGT	605	94 °C 30 s	57 °C 45 s	72 °C 45 s	²³
sta	GAAACAACATGACGGGAGGT	299	94 °C 30 s	57 °C 30 s	72 °C 30 s	²³
sta	GCACAGGCAGGATTACAACA	299	94 °C 30 s	57 °C 30 s	72 °C 30 s	²³
eaeA	ATGCTTAGTGCTGGTTTAGG	248	94 °C 30 s	51 °C 30 s	72 °C 30 s	²⁴
eaeA	GCCTTCATCATTTCGCTTTC	248	94 °C 30 s	51 °C 30 s	72 °C 30 s	²⁴
tsh	GGT GGT GCA CTG GAG TGG	620	94 °C 30 s	54 °C 40 s	72 °C 45 s	²⁵
tsh	AGT CCA GCG TGA TAG TGG	620	94 °C 30 s	54 °C 40 s	72 °C 45 s	²⁵
phoA	CGATTCTGGAAATGGCAAAAC	720	94 °C 30 s	60 °C 40 s	72 °C 1 min	²⁶
phoA	CGTGATCAGCCCTGACTATGAC	720	94 °C 30 s	60 °C 40 s	72 °C 1 min	²⁶
hly	AACAAGGATAAGCACTGTTCTGGCT	1177	94 °C 30 s	54 °C 40 s	72 °C 45 s	²⁷
hly	ACCATATAAGCGGTCATTCCCGTCA	1177	94 °C 30 s	54 °C 40 s	72 °C 45 s	²⁷
bla_KPC	ATGTCACTGTATCGCCGTCT	882	94 °C 1 min	55 °C 1 min	72 °C 1 min	²⁸
bla_KPC	TTACTGCCCGTTGACGCCC	882	94 °C 1 min	55 °C 1 min	72 °C 1 min	²⁸
bla_CTX	ATG TGC AGY ACC AGT AAR GTK ATG GC	593	94 °C 30 s	54 °C 40 s	72 °C 45 s	²⁹
bla_CTX	TGG GTR AAR TAR GTS ACC AGA AYC AGC GG	593	94 °C 30 s	54 °C 40 s	72 °C 45 s	²⁹
bla_TEM	ATCAGCAATAAACCAGC	516	94 °C 30 s	54 °C 40 s	72 °C 45 s	³⁰
bla_TEM	CCCCGAAGAACGTTTTC	516	94 °C 30 s	54 °C 40 s	72 °C 45 s	³⁰

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