Figure 2

Relative rate of [1-¹⁴C]C16:0 oxidation to CO₂ (A) or acid soluble products (ASP) (B), and BHB export (C) from primary bovine neonatal hepatocytes exposed to increasing concentrations of choline chloride (CC) and d,l-Methionine (DLM) and a 1 mmol/L fatty acid (FA) cocktail. Export of BHB was quantified after 24 h of treatment exposure and data were normalized to cellular DNA, and the average (± standard deviation) export of BHB for three cell preparations from the 0 μmol/L CC, 0 μmol/L DLM, 1 mmol/L FA treatment was 63.0 ± 21.2 nmol/µg DNA. Oxidation was quantified after 21 h of treatment exposure before a 3 h incubation with [1-¹⁴C]C16:0. The rate of [1-¹⁴C]C16:0 oxidation to CO₂ was expressed as pmol ¹⁴C substrate metabolized to ¹⁴CO₂·µg DNA⁻¹·h⁻¹ and the average (± standard deviation) rate for four cell preparations from the 0 μmol/L CC, 0 μmol/L DLM, 1 mmol/L FA treatment was 3.5 ± 0.9 pmol·µg DNA⁻¹·h⁻¹. The rate of [1-¹⁴C]C16:0 oxidation to ASP was expressed as pmol ¹⁴C substrate incorporated into ASP µg · DNA⁻¹·h⁻¹ and the average (± standard deviation) rate for four cell preparations from the 0 μmol/L CC, 0 μmol/L DLM, 1 mmol/L FA treatment was 3.0 ± 0.6 pmol·µg DNA⁻¹·h⁻¹. Data are expressed relative to the 0 μmol/L CC, 0 μmol/L DLM, 1 mmol/L FA treatment within each independent cell preparation. Values are least squares means, with SE represented by vertical bars. The P- values for contrast effects of CC and DLM are shown. Interactions between CC and DLM were not detected: P = 0.31, P = 0.27, and P = 0.27 for (A–C), respectively.