Figure 5

Consensus genome sequence of the Brucella canis outbreak strain from a kennel in São Paulo, Brazil. Shown are the two chromosomes of the B. canis BfR-SPBR-consensus genome derived from whole genome sequencing results of four B. canis isolates. The genomes were sequenced using Illumina NGS technology. The open reading frames (CDS) determined by Prokka v1.1289 are presented in the two outer circles with grey (+ strand) and black (-strand) boxes. Positions with nucleotide sequence variations in the B. canis BfR-SPBR-consensus genome compared to the reference strain B. canis ATCC 23365 are depicted in the inner circle and marked with the colors red (stop-loss variants), orange (missense variants), blue (upstream or downstream variants) and green (synonymous variants). Open reading frames affected by missense variants or stop-loss variants are shown in red together with the corresponding protein IDs given as NCBI numbers (WP_xxxxxxxxx.1).