Figure 3
Effect of CHOP overexpression on LCN2 expression by SubAB. (A) Control or FLAG-tagged CHOP plasmid-transfected HeLa cells were incubated for 24 h with mt SubAB or SubAB (400 ng ml−1). The lcn2 mRNA levels were measured by RT-qPCR as described in “Methods”. GAPDH was used as an internal control. Data are mean ± SD (n = 3). *P < 0.05, versus mt SubAB-treated control cells. Ns: not significant. (B) Cell lysates from 8 h or 24 h incubation with toxins were subjected to immunoblotting with the indicated antibodies. GAPDH served as a loading control. Experiments were repeated three times with similar results. ND: not detected.
