Figure 7
SubAB induces mouse LCN2 in RAW264.7 cells and mouse intestine. (A) RAW264.7 cells were incubated for 24 h with mt or wt SubAB. The mouse lcn2 (m lcn2) mRNA levels were measured by RT-qPCR as described in “Methods”. GAPDH was used as an internal control. Data are mean ± SD (n = 3). *P < 0.05, versus mt SubAB-treated control cells. (B) HeLa cells were transfected with control, FLAG-hLCN2 or FLAG-mLCN2, and then incubated for 24 h with control DMSO, 1 μg ml−1 TM, 400 ng ml−1 mt SubAB or wt SubAB. Cell lysates were subjected to immunoblotting with the anti-FLAG antibodies. GAPDH served as a loading control. Experiments were repeated three times with similar results. (C) Mouse duodenum, ileum and colon were collected from treated mice at the indicated time points after the intraperitoneal injection of SubAB (10 μg/mouse) (n = 3 for each group). The mlcn2 mRNA levels were measured by RT-qPCR as described in “Methods”. Mouse GAPDH (m gapdh) was used as an internal control. Data are mean ± SD (n = 3). *P < 0.05, versus mouse at 0 h.
