Figure 1
ICa in isolated SAN cells from wild-type and mutant mice. (A) Sample traces recorded from a HP of − 80 mV (top line) or from − 55 mV (bottom line) in SAN cells of wild-type (WT) (a), Cav3.1−/− (b), Cav1.3−/− (c) and Cav1.3−/−/Cav3.1−/− mice (d). (B) Current-to-voltage (I–V) relationship of Ca2+ current recorded from a HP = − 80 mV (black circles) or from HP = − 55 mV (red circles) in SAN cells from the following genotypes: wild-type (a, n = 16), Cav3.1−/− (b, n = 15), Cav1.3−/− (c, n = 15) and Cav1.3−/−/Cav3.1−/− mice (d, n = 18). In (a) and (b), the dashed line indicates the net ICaT I–V curve, calculated as the difference between values obtained from HP of − 55 mV from those from HP − 80 mV. (C,D) Relative density of ICaT and ICaL recorded in SAN cells isolated from wild-type (black bars, n = 16), Cav3.1−/− (a, open bars, n = 15), Cav1.3−/− (b, open bars, n = 15) and Cav1.3−/−/Cav3.1−/− mice (c, open bars, n = 18) at different test potentials from HP = − 80 mV (C) and HP = − 55 mV (D).
