Figure 8
Confocal line scan imaging of intracellular Ca2+ ([Ca2+]i) release during pacemaker activity of SAN cells from wild-type and mutant mice. Confocal line scan (left) images (top, left), corresponding sample traces of the time integral (bottom, left) and averaged frequency of spontaneous [Ca2+]i transients (right) of SAN cells form wild-type (A, n = 13), Cav3.1−/− (B, n = 8), Cav1.3−/− (C, n = 13) and Cav1.3−/−/Cav3.1−/− (D, n = 7) loaded with Fluo-4 and perfused with Tyrode’s solution (left) or IVA 3 µM (center) or IVA 3 µM + TTX 50 nM (right). Statistics: one-way ANOVA followed by Holm–Sidak multiple comparisons test. Whiskers indicate mean ± the SD.
