Table 1 Information on the 22 microsatellite markers for R. apiculata and the corresponding Genbank accession number.
Locus | Repeat motif | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) | Expected PCR product size | Genbank accession no |
|---|---|---|---|---|---|
RapT01 | (AT)10 | CCACACATTTTGCACCGAGC | CTCAATCGCCTCGGAATGA | 298 | MT364422 |
RapT02 | (CT)11 | TAAAGTGCGTGAATCCGCCA | ACTGCAACTCCACCGCTTAA | 138 | MT364423 |
RapT06 | (CT)14 | TCCTCCTTGCTCCAAGCTTG | ACATGGAGCCTGTAGCCAAA | 349 | MT364424 |
RapT08 | (GA)16 | TGATTCACTGCAAATGAGGCC | ACGAAACAAGCTTGCTCCCT | 102 | MT364425 |
RapT09 | (TC)16 | AGGCTCGGGATTTTCCTTCG | CTGGGAAGCATGGGGTAGTG | 170 | MT364426 |
RapT16 | (AG)11 | GGTGGATTGAGGGAAGTGGG | GGACACCTGGCAAACCCTTA | 296 | MT364427 |
RapT17 | (GA)18 | GTGGGAGCCTGCAAAGTTTG | GCGCGTTGAGCTCAGTTTAG | 281 | MT364428 |
RapT18 | (AT)11 | CACAGCAGTGTCATGGGCTA | AGGAGGAAGCTCATGCACAA | 165 | MT364429 |
RapT20 | (CT)12 | TCAACCGCCTTTTCCCTCTC | ACCTTAAGCAATCTCCGGCA | 102 | MT364430 |
RapT21 | (GA)15 | TATGTGGTCTGTGCAACCCC | GAAGCAGGCTGGATGGACTT | 156 | MT364431 |
RapT23 | (AGA)11 | TGGAGTGAAACACCTGATCCA | TGGGGCCAACAAAATGAGGA | 148 | MT364432 |
RapT25 | (ATT)11 | ATGCGACGTCACTCTGGTTT | CTCCTTCAGCTCTCCGCAAA | 267 | MT364433 |
RapT31 | (CAT)13 | TCGCTTAAGTCCCTGCCTTG | CCTTTGTAGGAGTGGCTGCA | 217 | MT364434 |
RapT38 | (AAT)10 | ACAGCCTTTACTCCAGCGAG | AGGTAAGGAACGCCGTGTTG | 347 | MT364435 |
RapT43 | (CAG)11 | GCATAGACAGGGCAGGGATC | CACCACGAGACCAAGTCTGA | 153 | MT364436 |
RapT46 | (CAG)12 | CCCAATCCGAACCCTAACCC | TGCTGCACATTCTGCCCTAA | 116 | MT364437 |
RapT51 | (AGAC)6 | TCTGAACCCGTTTTCTCCGG | GCCAGCAGCAAAATGAGGAC | 359 | MT364438 |
RapT53 | (TCTT)6 | CCGCAACGGTTCTTCAAAGG | GACCACAACAGTAGACAGCC | 338 | MT364439 |
RapT60 | (AAAG)9 | TCCACTTCGGTCTTTTCCCT | GGGCTAGAAGCGTCCGATTT | 369 | MT364440 |
RM111* | (TC)13 | AACCGTTACTCGCGTATGCT | CATTGCCTCCATTCCATT | 149 | AB721976 |
RM116* | (TA)12 | ATAAGACCATATGTAACACCCATTT | CCTCCTCTCATTCTTCATTTCA | 152 | AB721980 |
RM121* | (ATC)12 | TGGCCTATAGAGAAAGCGGA | CCTTCAATCCCAAACAGC | 179 | AB721982 |
