Figure 5
Representative H&E staining of Masquelet membrane (scale bar: 100 mm). H&E staining shown that the intensive fibrous, cell-rich, and vascularized tissue (white arrow) were formed in Fixation group (a) and Non-fixation group (c), while just little vessel like structure was observed in control group (e), in 4 weeks postoperatively. The micro-vessels had a tendency to increase and to be more mature in Fixation group (b) and Non-fixation group (d), while still little in control group (f), in 6 weeks postoperatively.
