Figure 2
From: ZNF354C is a transcriptional repressor that inhibits endothelial angiogenic sprouting
ZNF354C is a putative transcriptional repressor in HMEC-1. (a) Heat-map of RNA-Seq after overexpression of pCMV6-ZNF354C (ZNF354C) or pCMV6-Entry (CTRL). 3 replicates are shown. Top50 differentially expressed genes (DEG) were selected according to their p-value stringency (False discovery rate (FDR) < 0.05), logarithmic fold change (Log2FC > ± 0.585) and number of mean reads in both groups (> 5). Z-Score represents up- (red, positive value) or downregulation (blue, negative values) of genes after overexpression of ZNF354C. (b) Volcano plot of RNA-Seq after overexpression of pCMV6-ZNF354C (ZNF354C) or pCMV6-Entry (CTRL). Conditions are adjusted p-value < 0.05 (FDR); Colored points indicate genes significantly differentially expressed (log2FC > ± 0.5). Red, ZNF354C downregulated genes; green, ZNF354C upregulated genes; grey, non-regulated genes (log2FC < ± 0.5). (c) Percentage of DEGs harboring the NTCCAC, NNCCAC or none of these motifs in their promoter (0-500 bp upstream of the transcriptional start site). (d) RT-qPCR of the transcripts indicated after overexpression of pCMV6-ZNF354C in HMEC-1. PCMV6-entry was used as negative control (CTL) and set as 1. Data is normalised to b-actin. n = 3. Data are mean ± SEM. Paired t-test; *P < 0.05. (e, f) Gene ontology (GO) enrichment analysis with PANTHER for protein classes (e) or biological process complete (f) terms. Only downregulated DEG (logFC > 0.3) were used. Fisher’s exact test; FDR, false discovery rate.
