Figure 6
From: ZNF354C is a transcriptional repressor that inhibits endothelial angiogenic sprouting
Inhibition of endothelial sprouting by ZNF354C is dependent on the amino acids DV and MLE of the KRAB domain. (a–g) Spheroid outgrowth assay images (a) and quantification of sprout number (b, d, f) and cumulative sprout length (c, e, g) after overexpression of pCMV6-ZNF354C (354C), pCMV6-entry (NC) or the individual mutants DV (DV → AA), MLE (MLE → KKK) or EW (EW → AA). Cells treated under basal conditions (b, c) or with VEGF-A (d, e) or TNF-α (f, g) are shown. Scale bar indicates 50 µm. n = 20. One-Way ANOVA with Bonferroni post-hoc test. *P < 0.05.
