Figure 4 | Scientific Reports

Figure 4

From: Platycodin D enhances LDLR expression and LDL uptake via down-regulation of IDOL mRNA in hepatic cells

Figure 4The alternative text for this image may have been generated using AI.

PG and PD increase LDLR protein levels by inactivation of IDOL mRNA in hepatic cell lines. (A) SNU-387 and Hep3B cells were treated with 2.5 μM PD and 250 μg/mL PG for 24 h. Cell lysates were analyzed by western blotting with anti-LDLR and anti-GAPDH antibodies. (C) Hepatic cells were co-transfected with and pLDLR-Luc and pRL-TK vector for 24 h followed by treatment with 2.5 μM PD and 250 μg/mL PG for 24 h. Luciferase activity was measured and normalized by Renilla luciferase expression. (B, D) Hepatic cells were treated with 2.5 μM PD and 250 μg/mL PG for 24 h. LDLR (B) and IDOL (D) mRNA expression was analyzed by real-time PCR. The data represent mean ± SD of three independent experiments. *P < 0.05 by one-way ANOVA with Tukey’s post hoc test.

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