Table 4 Formulation and proximate compositions of the experimental diets (% of dry matter).

From: Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein

Ingredients

Diets

C0

C8

C12

C16

C20

C24

Fish meal

57

57

57

57

57

57

Wheat gluten

13

13

13

13

13

13

Alpha-starch

0

5

5

5

5

5

Corn starch

0

1.7

5.7

9.7

13.7

17.7

Soybean lecithin

1

1

1

1

1

1

Fish oil

3.5

3.5

3.5

3.5

3.5

3.5

Choline chloride

0.4

0.4

0.4

0.4

0.4

0.4

Ethoxyquin

0.05

0.05

0.05

0.05

0.05

0.05

Mold inhibitora

0.1

0.1

0.1

0.1

0.1

0.1

Monocalcium phosphate

0.5

0.5

0.5

0.5

0.5

0.5

Vitamin premixb

0.6

0.6

0.6

0.6

0.6

0.6

Minerals premixc

0.5

0.5

0.5

0.5

0.5

0.5

Y2O3

0.1

0.1

0.1

0.1

0.1

0.1

Bentonite

6.7

0

0

0

0

0

Carboxymethyl cellulose

16.55

16.55

12.55

8.55

4.55

0.55

Total

100

100

100

100

100

100

Proximate analysis (% dry matter)

Dry matter (% diet)

95.65

96.80

96.61

97.23

97.08

96.96

Crude protein

49.96

49.59

49.49

49.60

49.99

50.44

Crude lipid

9.59

9.73

9.65

9.98

9.75

9.96

Carbohydrated

0.93

7.01

11.06

15.60

19.96

23.18

Ash

17.73

12.46

11.72

11.73

11.93

12.13

  1. aMold inhibitor: 50% calcium propionic acid and 50% fumaric acid.
  2. bVitamin premix (g kg−1 of diet): microcrystalline cellulose, 9.884 × 10–2; VA, 1.92 × 10–4; VB1, 1.5 × 10–4; VB2, 2.7 × 10–4; VB6, 1.2 × 10–4; VB12, 6 × 10–5; VD, 2.1 × 10–4; VE, 1.44 × 10–3; VK, 6 × 10–5; calcium pantothenate, 3.6 × 10–4; nicotinic acid, 1.2 × 10–3; folic acid, 1.2 × 10–4; biotin, 3.6 × 10–4; inositol, 4.8 × 10–3; VC phosphate, 1.2 × 10–2.
  3. cMineral premix (g kg−1 of diet): MgSO4·7H2O, 6 × 10–3; CuSO4·5H2O, 5 × 10–5; FeSO4·H2O, 4 × 10–4; ZnSO4·H2O, 2.5 × 10–4; MnSO4·H2O, 2.25 × 10–4; CoCl2·6H2O (1%), 2.5 × 10–4; Na2SeO3 (1%), 1 × 10–4; calcium iodate, 3 × 10–4; zeolite powder, 4.243 × 10–2.
  4. dDetermined by the 3,5-dinitro salicylic acid method120.