Figure 1

Derivation of trophoblast stem cells and primary syncytiotrophoblasts from villous cytotrophoblasts. (A) Primary villous cytotrophoblasts (pri-CTB) were isolated from macaque placentas and cultured in trophoblast stem cell (TSC) medium to support cellular proliferation or differentiated to primary syncytiotrophoblasts (pri-ST). (B) Phase contrast images of TSC and pri-ST at 24, 48 and 72 h in culture. Scale bars represent 100 µm. (C) Representative phase-contrast microscopic images of TSCs at passage (p) 1, 2 and 3. Scale bars represent 500 µm.