Figure 4

DNA methylation of ELF5 and C19MC gene regions. Methylation was evaluated in macaque blastocyst stage embryos (embryos 1–3), TSC lines, BeWo trophoblasts, and rhesus macaque fetal fibroblasts. (A) ELF5 methylation at 11 CpG sites for each cell type. Circles are colored to indicate the proportion of clones either methylated (blue) or unmethylated (orange) at each CpG site. (B) C19MC methylation at 34 CpG sites represented as the number of clones with either < 25% or > 60% methylation. The number of clones analyzed is indicated in brackets above each bar. All clones analyzed were either < 25% or > 60% methylated, representing an either hypo- or hyper-methylated allele, respectively.