Figure 5

Differentiated trophoblast display protein markers of syncytium formation. (A) Representative observations for immunocytochemistry against KRT7, Vimentin, AP2-γ, mCG and Mamu-AG is represented in TSC, ST-2D, ST-3D and rhesus fetal fibroblasts. For all panels, the specific marker of interest is shown in red and the nuclei are stained with DAPI in blue. Scale bar denotes 100 µM. Secretion of mCG (B) and progesterone (C) by TS, ST2D, ST3D, and EVT cells was normalized to the amount of protein. Data is represented as the mean ± the standard error of the mean (SEM). Data for rhesus macaque cells is indicated by circles, whereas data for cynomolgus macaque cells is indicated by a triangle. Significance was determined using a Kruskal–Wallis test with Dunn’s correction.