Figure 7

TSC gene expression evaluated by scRNA-Seq. (A) Identification of individual p2 and p10 TSCs of rh121118 within the t-SNE distribution with the number of individual cells sequenced for each passaged denoted in parentheses. (B) Gene expression by K-means clustering analysis of combined p2 and p10 cells. The number of significantly up or down regulated genes in this and panel C appear in parentheses next to the cluster number. (C) Clustering of p2 and p10 cells upon analysis of gene expression levels using the LMO algorithm. Individual p2 and p10 cells from the combined analysis are shown to the right. Clusters where cells were more highly represented from one of these two passages are indicated by gray circles.