Figure 5 | Scientific Reports

Figure 5

From: Epstein–Barr virus peptides derived from latent cycle proteins alter NKG2A + NK cell effector function

Figure 5

EBV peptides induce specific killing by NK cells expressing NKG2A. (a) HLA-E stabilization after peptide loading. (b) Comparison of target cell killing when loaded with EBV peptides at E:T ratios of 3:1. HLA-A03 leader peptide and BLZF1 peptide were used as controls. The percentage of cytotoxicity after co-culture of 721.174 cells loaded with EBV peptides and sorted NKG2A + /-NKG2C- cells was calculated with the following formula: [(Control − Test)/Control] × 100 = % of Cytotoxicity. Data shown is representative of 3 donors. One-way ANOVA comparing HLA-A03 leader peptide with all different conditions (***p = 0.0001, ****p < 0.0001). No significant differences were observed in the NKG2A-2C-cells. (c) Gating strategy allowing the distinction between live cells (Live/Dead negative) and dead cells (Live/Dead negative).

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