Figure 1

mafr-1 (KO) produces fertile, viable hermaphrodites. (a) Schematic diagram showing region deleted in mafr-1(syb557), a CRISPR-generated and sequence confirmed deletion of the entire mafr-1/C43H8.2 coding sequence from the start to stop codons, hereafter referred to as mafr-1 (KO) (Adobe Illustrator v24.3). (b) Developmental time course of WT and mafr-1 (KO) hermaphrodites. Each line represents one population of > 100 animals. (c) Lifespan survival assay of WT and mafr-1 (KO) hermaphrodites. Individual lines represent average of two or three biological replicates of 50-worm populations (see Fig. S1a). No significant difference was found by Log-rank (Mantel-Cox) test. (d) Quantitative PCR expression analysis of mafr-1 and three tRNA RNA pol III transcripts: initiator Methionine, Tryptophan, and Iso-leucine. (e) Lipid abundance as measured by Nile Red staining intensity. (f) Quantitative PCR expression of vit-2 and vit-4. (g) Total brood size of WT and mafr-1 (KO) hermaphrodites (n > 10). (h) Time course showing reproductive output of WT and mafr-1 (KO) hermaphrodites throughout reproductive span. Unless otherwise specified, statistical comparisons made by Student’s t-test (two-tailed). ns = no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.