Figure 3
mafr-1 (KO) males have diminished sperm quality. (a) Spermatid size in WT and mafr-1 (KO) males. See Supplemental Data Set for further statistical analysis. (b) Spermatid size of day 2 adult WT and mafr-1 (KO) males following RNAi knockdown of brf-1. See Supplemental Data Set for further statistical analysis. (c) In vitro Pronase activation of WT and mafr-1 (KO) male spermatids. (d) In vitro Pronase activation of day 2 adult WT and mafr-1 (KO) males following RNAi knockdown of brf-1. (e) Schematic diagram showing setup of male sperm competition mating assay (Adobe Illustrator v24.3). (f) Proportion of progeny fertilized by self-sperm from WT- and mafr-1 (KO)-mated hermaphrodites. Experiments done in biological triplicate. Statistical comparisons made by Student’s t-test (two-tailed). ns = no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
