Figure 4
Germline expression of WT mafr-1 rescues sperm quality phenotypes. (a) Spermatid size in WT, mafr-1 (KO), and germline-specific rescue males. See Supplemental Data Set for further statistical analysis. (b) In vitro Pronase activation of WT, mafr-1 (KO), and germline-specific rescue male spermatids. mafr-1 (KO)/Rescue comparison done via unpaired, one-tailed t-test. Experiments done in biological triplicate. (c) Proportion of progeny fertilized by self-sperm from WT, mafr-1 (KO), and germline-specific rescue-mated hermaphrodites. WT and mafr-1 (KO) data are same data presented in Fig. 3e. (d) Model of effects of MAFR-1 on spermatid quality. MAFR-1 interacts with MSP-53, SSS-1, and F48C1.6, and its repression of RNA pol III is required for proper sperm maturation (Adobe Illustrator v24.3). Unless otherwise specified, statistical comparisons made by Student’s t-test (two-tailed). ns = no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
