Figure 5 | Scientific Reports

Figure 5

From: Deviant reporter expression and P2X4 passenger gene overexpression in the soluble EGFP BAC transgenic P2X7 reporter mouse model

Figure 5

sEGFP-positive cells in the hippocampus and sporadic sEGFP expression in Purkinje cells. Brain slices from BAC transgenic sEGFP mice were co-labelled with an anti-GFP antibody and antibodies against marker proteins for neuronal and glial cell types. Representative immunofluorescence stainings are shown. (A) Immunofluorescence staining in the CA3-CA2 border with antibodies against calbindin-1 and calretinin (GABAergic interneurons) and in the CA3 region with an antibody against ZnT3 (synaptic vesicles). (B) Staining in the cerebellum with antibodies against Calbindin-1 (Purkinje cell) and S100β (Bergman Glia). Single EGFP-positive cells show Purkinje cell morphology (white arrowheads). Note that not all Purkinje cells are EGFP-positive (black arrowhead). White arrows indicate an unidentified cell type in the molecular layer. (C) Staining in the cerebellum with antibodies against NG2 (oligodendrocyte progenitor cells) and parvalbumin (Purkinje cells and molecular layer interneurons). Examples of double-positive cells are pointed out using white arrowheads in each case. DAPI staining is shown in blue. CA, cornu ammonis; ML, molecular layer; GR; granular layer. Scale bar: 50 μm.

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