Table 2 PCR primer sequences.
From: Coffee decoction enhances tamoxifen proapoptotic activity on MCF-7 cells
Era | 5′-AGCACCCTGAAGTCTCTGGA-3′ (upstream) |
5′-GATGTGGGAGAGGATGAGGA-3′ (downstream) | |
c-Myc | 5′-CCCTCCACTCGGAAGGACTA-3′ (upstream) |
5′-GCTGGTGCATTTTCGGTTGT-3′ (downstream) | |
TFF1 | 5′-CCCAGTGTGCAAATAAGGGC-3′ (upstream) |
5′-TGGAGGGACGTCGATGGTAT-3′ (downstream) | |
CTSD | 5′-TTCATCGGCCGCTACTACAC-3′ (upstream) |
5′-CTGCTCTGGGACTCTCCTCT-3′ (downstream) | |
GREB1 | 5′-GCACATCTTGTACTTTGATGCCC-3′ (upstream) |
5′-TGTCCTCAGCTGCAGTTTGTT-3′ (downstream) | |
PgR | 5′-CGGTCCAGCCACATTCAACA-3′ (upstream) |
5′-GTCATGACGACTGGACTCCC-3′ (downstream) | |
p21 | 5′-CACCACTGGAGGGTGACTTC-3′ (upstream) |
5′-CGTGGGAAGGTAGAGCTTGG-3′ (downstream) | |
PUMA | 5′-AAATTTGGCATGGGGTCTGC-3′ (upstream) |
5′-CCTCTTGTCTCACAAATCTGGC-3′ (downstream) | |
BAX | 5′-CCAGCTCTTTAATGCCCGTTC-3′ (upstream) |
5′-CAGTCGCTTCAGTGACTCGG-3′ (downstream) | |
BAX | 5′-ACTCCACTCACGGCAAATTC-3′ (upstream) |
5′-CCTTCCACAATGCCAAAGTT-3′ (downstream) |
