Figure 6
PCR-based detection of LpFTRL-related gene from retail products. (a) Retail products of barley (i), wheat (ii), oat (iii), and rice (iv). (b) PCR assay results were visualised on an agarose gel, using the primers designed for LpFTRL. The size (bp) of PCR amplicons is indicated on the right side of each image. As a control experiment, PCR primers for the florigen candidate gene (FT in wheat and barley, and Hd3a in rice) were used. For demonstration of absence of Epichloë and Claviceps species in the retail products, PCR primers specific to the fungal species (EfMCF_F and R, and C.purpurea_D0288F and D0289R) were used. The gDNA samples from the perennial ryegrass genotype Impact04 and E. festucae were used, as positive controls for amplification with the plant and fungus-specific primers, respectively. With the PCR primers specific to Claviceps species, amplification from E. festucae gDNA template was observed, presumably due to sequence similarity between Epichloë and Claviceps species. ‘NTC’ stands for ‘no DNA template control’.
