Figure 4
P2Et extract decrease the viability of mammospheres derived from breast cancer patients after in vitro treatment. (a) Representative confocal microscopy image of the effect of P2Et extract, DX or DX + P2Et on the mammospheres viability of breast cancer patients. Luminal A (LA), Luminal B (LB), Triple negative (TN) and Her2. Calcein AM (green), Ethidium homodimer-1 (Red). (b) Cell death percentage in mammospheres by molecular subgroups of breast cancer patients after treatment. (c) Cell death percentage in all mammospheres from breast cancer patients after treatment. Data are presented as violin plots and each point represents an independent sample; dotted lines indicate quartiles. Exact p-values were calculated using One-way ANOVA with Tukey post-test analysis. (d) Spearman nonparametric correlation between percentage of cell death with ABCB1, ABCG2, ABCC1 gene in mammospheres from breast cancer patients after P2Et, DX or Ethanol (negative control) treatment, coefficient of determination r and exact p-value are shown. *p < 0.05; **p < 0.01; ***p < 0.001.
