Figure 1 | Scientific Reports

Figure 1

From: Recovery of motor function of chronic spinal cord injury by extracellular pyruvate kinase isoform M2 and the underlying mechanism

Figure 1

VCP is a target protein of extracellular PKM2. (a) Identification of VCP as a target of PKM2 by DARTS and western blot. Plasma membrane fraction of mouse cortical neurons were lysed, and recombinant PKM2 or vehicle solution was incubated with lysates for 30 min at room temperature. The mixture was proteolysed using thermolysin for 60 min at 37 °C and electrophoresed for western blotting for VCP. (b) Quantified band intensities of VCP. Student’s unpaired t-tests. **p < 0.01, n = 3. (c) A standard curve of VCP ATPase activity. (d) Inhibitory effect of CB-5083 on VCP ATPase activity. Used VCP concentration was 200 nM. One-way ANOVA with post hoc Dunnett’s test. *p < 0.05, **p < 0.01 vs. 0 nM, n = 3. (e) Effect of PKM2 on VCP ATPase activity. Used VCP concentration was 200 nM. **p < 0.01 vs. vehicle, n = 5–6.

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