Figure 4
From: Alterations in detrusor contractility in rat model of bladder cancer
Bladder cancer up-regulates DSM contractility dependent on activation of heat and capsaicin-sensitive TRPV1-channel. (a,b) Representative original recordings of normal (control, a) and cancerous (b) DSM strips contractions in response to application of TRPV1 agonist capsaicin (CAP, 10 µM) on the background of atropine (ATR, 1 µM); upward spikes are EFS-contractions; note, development of stronger tension and stronger increase of EFS-contractions in cancer (b) vs. normal (a) DSM in response to CAP; in these as well as other original recordings from subsequent figures the first upward spike—contraction in response to KCl (60 mM) used for data normalization, dashed line—the level of baseline tension, thick solid lines—markers of drugs applications. (c,d) Quantification of the changes in tension (c) and amplitude of EFS-contractions (AEFS, d) at indicated interventions in normal (Ctrl, light grey bars, mean ± SD, n = 8) and cancerous (Cncr, dark grey bars, mean ± SD, n = 10) DSM strips; “*” significant difference (P < 0.05) between corresponding values; note, development of stronger tension and AEFS enhancement in response to CAP in cancer vs. control DSM.
