Figure 4
From: Distinct regional ontogeny and activation of tumor associated macrophages in human glioblastoma
Single cell trajectory analysis and pseudotime ordering. (A) Tree structure of tumour core immune cells, coloured by pseudotime, MPI, and AMDI (right to left). Note that there are two branches (labeled 1 and 2) and five states (lines), labelled numerically in the leftmost plot. These states are the result of the unsupervised DDRTree algorithm and represent the evolution pathway of immune cells. Mean scores for each state are labelled in the MPI and AMDI-annotated plots, and an arrow is used to demonstrate the direction of numeric increase. (B) The same process is repeated for the peripheral immune cells, whose tree structure also has 2 branches and 5 states. (C,D) Correlation of mean state-specific AMDI and MPI scores in tumour core (C) and periphery (D). For each state in the tree structures, mean AMDI and MPI value is plotted, with dot size proportional to the number of cells in that state and colour representing the state’s mean pseudotime value. Numbers correspond to the state labels from the leftmost plots in (A,B).
