Figure 1
From: Integrative quantitative-phase and airy light-sheet imaging
(a) A representative 3D fluorescent image of 500 nm diameter particles using Airy beam illumination after deconvolution. (b) The same particles as in (a) displayed by QPI; inset depicts the scale bar in radians. (c) Schematic illustration of the imaging set-up comprising of two objectives arranged orthogonally, one for detection (63x/0.7) and one (20x/0.42) for guiding the Airy beam to a sample enclosed in a microfluidic system; the Airy beam is generated by a spatial light modulator (SLM) and scanned in the y direction. A 0.55-NA condenser guides the white illumination on to the sample, and the transmission is encoded by a second SLM to reconstruct the optical-phase image. RDF stands for reflecting dichroic filter, which directs the fluorescent signal to sCMOS1 and the transmitted white light to a second SLM and sCMOS2.
