Table 1 Primers used to create the studied PfCCT constructs.

From: Identification of a nuclear localization signal in the Plasmodium falciparum CTP: phosphocholine cytidylyltransferase enzyme

Primer name

Sequence (5′–3′)

C1-for

CGAGCTAGCATGATCATCAAAGTGAACAGCG

C1-rev

CGATCTCGAGCTATCCACGTTGCAGGCTACGTTCG

C1M1-rev

CGATCTCGAGCTATTTTTTTTTTTTCAGTTCATCTTTCATGC

C2-for

TCCGCTAGCATGGTCGACCCGGATACCAATC

SV40-NLS-C2 for

CAGCGCTAGCATGGGAGCTTCACCCAAGAAGAAGAGAAAG

GTGGGTCGACCCGGATACCAATC

C2-rev

GACCTCGAGTTATAGCTCATTCGGGTGGATG

C2M2-rev

CACGCTCGAGTCAGCTGCTGGTTTCATCATC

C1ΔK for

CCGTACGCCAATAACCAGAAAGAAGATATCTACGCCTGGCTG

C1ΔK rev

CCGTACGCCAATAACCAGAAAGAAGATATCTACGCCTGGCTG

C2ΔK for

AACAATCAGAAAGAAGATATTTATGCTTGGCTGAAACG

C2ΔK rev

AATATCTTCTTTCTGATTGTTAGCATACGGGATGTC

  1. Bold letters indicate the restriction sites of NheI and XhoI for the forward and reverse primers, respectively. Italic letters show the overlapping regions. Underscore shows the Simian Virus 40 NLS sequence that was used to generate the NLS-tagged construct (NLS C2M2). Lysine-rich loop deleted (ΔK) constructs were generated by QuikChange site-specific deletion59.
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