Figure 1 | Scientific Reports

Figure 1

From: Endoplasmic reticulum chaperone BiP/GRP78 knockdown leads to autophagy and cell death of arginine vasopressin neurons in mice

Figure 1

Validation of rAAV vectors and BiP shRNA in mouse AVP neurons. (A) Representative images of immunofluorescence staining to enhance the Venus signal (green), and to detect AVP (magenta) and OT (cyan) in the SON 2 weeks after rAAV-AVPp-Venus injection. Scale bar: 50 μm. (B) The proportion of both AVP and Venus-immunoreactive (ir) cells to AVP-ir (AVP + Venus + /AVP +) and Venus-ir cells (AVP + Venus + /Venus +) in the SON 2 weeks after rAAV-AVPp-Venus injection. (C) Representative images of in situ hybridization for BiP mRNA in the SON and PVN 2 weeks after injection of rAAV-AVPp-scrambled shRNA (cont sh 2wk) and rAAV-AVPp-BiP shRNA (BiP sh 2wk). Scale bars: 100 μm. (D) Expression levels of BiP mRNA in the SON and PVN in the cont sh 2wk and BiP sh 2wk groups. Mean expression levels of BiP mRNA in the SON and PVN in the cont sh 2wk group are expressed as 100. Results were analyzed by an unpaired Student’s t-test and are expressed as the means ± SE (B, n = 6; D, n = 7 per group).

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