Table 3 List of primers and probes used for the multiplex dPCR assay.
Name | Description | Oligonucleotide Sequence (5′ > 3′) |
|---|---|---|
SMN1f | Forward primer | 5′-AATGCTTTTTAACATCCATATAAAGCT-3′ |
SMN1r | Reverse primer | 5′-CCTTAATTTAAGGAATGTGAGCACC-3′ |
SMN1p | Probe | 5′-FAM-TCCTTACAGGGTTTCAGACAAAATCAA-QSY-3′ |
SMN2f | Forward Primer | 5′-AATGCTTTTTAACATCCATATAAAGCT-3′ |
SMN2r | Reverse Primer | 5′-CCTTAATTTAAGGAATGTGAGCACC-3′ |
SMN2p | Probe | 5′-VIC-TCCTTACAGGGTTTTAGACAAAATCAA-QSY-3′ |
totalSMNf | Forward primer | 5′-GGAAGTTTCAGGAAGTGGTAGG-3′ |
totalSMNr | Reverse primer | 5′-CCACCAGGACTGCCTTTATATC-3′ |
totalSMNp | Probe | 5′- TYE675-AGAAGATGGCAGGGTGTTGGGAAT-IAbRQSp-3′ |
RPPH1f | Forward primer | 5′-CTTTGCCGGAGCTTGGA-3′ |
RPPH1r | Reverse primer | 5′-GAGAGTAGTCTGAATTGGGTTATGA-3′ |
RPPH1p | Probe | 5′-6-TAMRA-ACCTCACCTCAGCCATTGAACTCAC-IAbRQSp-3′ |
